The PetakaG3 from Celartia in Spain incorporates novel technology to create the ideal environment for cell culture. This compact and easy to use product (please see dimensions below) features a resealable access port which keeps the interior virtually hermetically closed to external elements in order to eradicate culture contamination and cross-contamination. It also features a gas transfer quenching system that allows gas exchange without gas bubbling and a filtered exit vent to prevent atmospheric contact.
The unit's shape and size make it comfortable and easy to handle, easy to store and trouble-free and safe to transport. The PetakaG3 design allows very low amounts of carbon dioxide to move in an out of the device through an 0.2 mm pore filter. No additional carbon dioxide is therefore required and cells in the PetakaG3 can be incubated in a closed and stable environment in regular incubators (without humidification) up to the limit of the cells' own metabolism.
There are five versions of the PetakaG3:
- PetakaG3™ LOT (Low Oxygen Transfer
- PetakaG3™ ET™ (Easy Transfer)
- PetakaG3™ HOT (High Oxygen Transfer)
- PetakaG3™ FLAT Suspended Cell Culture)
- PetakaG3™ MATRIX (Specific Molecular Matrix)
The PetakaG3 HOT, FLAT and MATRIX are manufactured from a co-polymer called Styrolux and are specifically designed for high oxygen respiring cells such as liver cells, monoclonal hybridomas, etc. Cells can be put into dormancy for transportation or short-term storage but the high gas diffusion material of the PetakG3 HOT does not allow the oxygen level to go below 2%; it is therefore unsuitable for long-term dormancy (more than 30 days without media change). Culturing for more than two weeks is also not recommended as the PetakaG3 walls not only allow gas to diffuse through but they also allow minute amounts of water to escape.
There are five versions of the PetakaG3:
- PetakaG3™ LOT (Low Oxygen Transfer
- PetakaG3™ ET™ (Easy Transfer)
- PetakaG3™ HOT (High Oxygen Transfer)
- PetakaG3™ FLAT Suspended Cell Culture)
- PetakaG3™ MATRIX (Specific Molecular Matrix)
The PetakaG3 HOT, FLAT and MATRIX are manufactured from a co-polymer called Styrolux and are specifically designed for high oxygen respiring cells such as liver cells, monoclonal hybridomas, etc. Cells can be put into dormancy for transportation or short-term storage but the high gas diffusion material of the PetakG3 HOT does not allow the oxygen level to go below 2%; it is therefore unsuitable for long-term dormancy (more than 30 days without media change). Culturing for more than two weeks is also not recommended as the PetakaG3 walls not only allow gas to diffuse through but they also allow minute amounts of water to escape.
The PetakaG3 LOT and ET are manufactured from a crystal polystyrene called Styron and are designed for most mammalian cells with a low to medium oxygen diffusion rate such as CHO cells. This is the material from which all previous versions of the Petaka were manufactured. This material allows up to two months of dormancy without media change of low/medium oxygen respiring cells and up to several months with media change. Culturing in PetakaG3 LOT brings the dissolved oxygen levels in the media down to 1.5% that induces dormancy at confluence.
More Information
Visit Discovery's main instrumentation website for complete details on the PetakaG3 system. If you are interested in trying out the PetakaG3 in your lab then contact our Customer Service Department to request free samples.
